Oxygen Limitation within a Bacterial Aggregate

Oxygen Limitation within a Bacterial Aggregate

ABSTRACT Cells within biofilms exhibit physiological heterogeneity, in part because of chemical gradients existing within these spatially structured communities. Previous work has examined how chemical gradients develop in large biofilms containing >10(8) cells. However, many bacterial communities in nature are composed of small, densely packed aggregates of cells (≤ 10(5) bacteria). Using a ge...

A Column Bioleaching Model for Chalcocite: An Investigation of Oxygen Limitation and Bacterial Inoculation on Leaching

Oxygen Limitation and Carbon Dioxide Inhibition

The growth of meat-spoiling Pseudomonasfragi 72 was studied on a defined salt medium supplemented with L-aspartate, citrate, creatine, creatinine, D-glucose, L-glutamate, and L-lactate. The utilization of the different carbon sources was followed in batch and continuous culture and under the influence of oxygen limitation and carbon dioxide inhibition (50% CO2 in air). Under nonrestricted atmos...

Lung metastatic load limitation with hyperbaric oxygen.

Despite some theoretical concern about cancer-enhancing effects ofhyperbaric oxygen (HBO2) therapy, it is frequently administered to cancer patients. We evaluated the growth of murine breast cancer cells in the lung after hyperbaric oxygen treatment in an experimental metastasis assay. Young nu/nu mice were injected intravenously with 3 x 10(3) 4T1-GFP tumor cells per g body weight followed by ...

Temperature-dependent oxygen limitation in insect eggs.

Most terrestrial insect embryos support metabolism with oxygen from the environment by diffusion across the eggshell. Because metabolism is more temperature sensitive than diffusion, embryos should be relatively oxygen-limited at high temperatures. We tested whether survival, development time and metabolism of eggs of a moth, Manduca sexta, were sensitive to experimentally imposed variation in ...